EL-C1600N100013-B
Test - lowercase jpg
Kaixin micro test
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The ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA), followed by incubation and washing to remove unbound enzyme conjugates, followed by the addition of substrates A, B, and the enzyme conjugate. Produce color. ELISA kit composition: 1, serum: avoid any cell stimulation during the operation. Use tubes without pyrogens and endotoxins. After collecting the blood, the red blood cells were quickly and carefully separated by centrifugation at 1000 x g for 10 minutes. 2. Plasma: EDTA, citrate, heparin plasma can be used for detection. The pellet was removed by centrifugation at 1000 x g for 30 minutes. 3. Cell supernatant: Centrifuge at 1000 xg for 10 minutes to remove particles and polymer. 4. Tissue homogenization: The tissue is added to the appropriate amount of physiological saline and chopped. Centrifuge at 1000 xg for 10 minutes and take the supernatant. 5. Storage: If the sample is not used immediately, it should be divided into small parts - 70 ° C to avoid repeated freezing. Do not use hemolysis or hyperlipemia as much as possible. ELISA kit component 1 pre-coated plate: anti-mouse interleukin-6 rabbit IgG, affinity purified 96T 2 enzyme-labeled antibody: (30 times concentrated) HRP-labeled anti-mouse interleukin-6 rabbit IgG, affinity purification 0.4mL x 1 3 Standard: Recombinant Mouse Interleukin-6 0.5mL x 2 4 EIA Buffer: Contains 1% BSA, 0.05% Tween 20 BPS 30mL x 1 5 Labeled Antibody Diluent: Contains 1% BSA, 0.05% Tween 20 BPS 12mL x 1 6 Reagent: TMB substrate 15mL x 1 7 Stop solution: 1N sulfuric acid 12mL x 1 8 Concentrated washing solution: (40 times concentrated) with 1% BSA, 0.05% Tween 20 BPS 50mL x 1 Description 1 Equipment required for the experiment (but not provided in the kit) Microplate reader (450nm) Micropipette and its nozzle cylinder and beaker deionized water refrigerator (4 ° C) Coordinate paper (log / log) Absorbent paper test tube ( For standard dilution) Incubator (37 ° C ± 1 ° C) Wash bottle (for washing) Disposable reagent tube (for concentrated enzyme labeled antibody and color developer)
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