The kit uses a double antibody sandwich assay to determine the level of mouse fibroblast growth factor-21 (FGF-21) elisa kit in the specimen. The microporous plate was coated with purified mouse fibroblast growth factor-21 (FGF-21) antibody to prepare a solid phase antibody, and fibroblast growth factor-21 (FGF-) was sequentially added to the microcapsules of the coated monoclonal antibody. 21), and then combined with HRP-labeled fibroblast growth factor-21 (FGF-21) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which was thoroughly washed and then added with a substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with fibroblast growth factor-21 (FGF-21) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of mouse fibroblast growth factor-21 (FGF-21) in the sample was calculated from a standard curve.
1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity.
Bitcoin mining machines are one way to obtain bitcoins. Bitcoin is an online virtual currency created by open-source peer-to-peer software. Instead of being issued by a specific currency institution, and produced by the massive computation of a specific algorithm, the Bitcoin economy uses a distributed database of nodes throughout the P2P network to identify and record all transactions. The decentralized nature and algorithm of P2P can ensure that it is impossible to artificially manipulate the value of bitcoin by producing a large number of coins.
Any computer can become a mining machine, but the returns will be low, and it may not mine a single bitcoin in a decade. Many companies have developed specialized Bitcoin mining machines with special mining chips that are tens or hundreds of times faster than normal computers.
Currency system by the user (the user through the key control wallet), trade (trade will be broadcast to the whole currency network) and miners (generated by competition calculation agreed on each node of the chain block, block chain is a distributed public authority books, contains the currency network of all the transactions happened)
Bitcoin miners manage the Bitcoin network by solving the problem of proof-of-work mechanisms with a certain amount of work -- confirming transactions and preventing double payments. Because hashing is irreversible, finding the number of random adjustments required to match is difficult, requiring a trial-and-error process that can predict the total number of times. This is where the proof-of-work mechanism comes into play. When a node finds a solution that matches the requirement, it can broadcast its result to the whole network. Other nodes can then receive the newly solved data block and check whether it matches the rule. If the other nodes calculate the hash value and find that the requirement (the operation goal required by Bitcoin) is satisfied, then the data block is valid and the other nodes accept the data block.
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