Determination of Berberine Hydrochloride in Weikangning Tablets by UV-1500PC Ultraviolet Spectrophotometry - Master's thesis

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Determination of Berberine Hydrochloride in Weikangning Tablets by UV-1500PC Ultraviolet Spectrophotometry

Key words: ultraviolet spectrophotometry; content of berberine hydrochloride in Weikangning tablets; US analysis ;

$Width="753" height="849" src="http://i.bosscdn.com/blog/nophoto.gif" /></p>Abstract: Study on the Determination of Berberine Hydrochloride in Weikangning Tablets method. The content was determined by ultraviolet spectrophotometry, using 375 nm as the measurement wavelength and 0.05 mol/L sulfuric acid as the control solution. Results: The berberine hydrochloride showed a good linear relationship between 4 and 28 mg/L. The correlation coefficient was r=0.9999, the average recovery was 99.55% (n=5), and the RSD was 0.36%. Conclusion: This method is simple, accurate, and has good recovery and reproducibility. It is suitable for rapid analysis in the production process. Determination of the content of berberine hydrochloride of weikangning tablets by UV spectrophotometry ABSTRACT: 0BJECTIVE T0 develop fin assay method for the determination of berberine hydrochlonide in weikangning tablets. METHOD UV spectrophotometry was selected to determine the content and the 0.05 mol/L sulfuric acid was used as the reference solution. The absorbance was measured at 375 nm. RESULTS The calibration curve for berberiny hydrochloride was linear within the range of 4-28 mg/L (r=0.9999), The average recovery was 99.55% (n=5), and the RSD was 0.36%. CONCLUSIONS The method was proved to be simple,precise and reproducible. It is especial1y practicable for the-process analysis. Weikangning tablets are mainly used for the treatment of peptic ulcers, and the curative effect is certain. Berberine hydrochloride is the main component of the preparation, and the method for determining the content of the raw materials and tablets is contained in the second part of the Chinese Pharmacopoeia 2000 edition, which is a redox method, and the operation is cumbersome. It has been reported in the literature in China to determine its content by ultraviolet spectrophotometry. In this paper, the method for determining the content of berberine hydrochloride, the main component in the preparation, was established by UV spectrophotometry. It is easy to operate and provides a practical method for quality control, and its measurement conditions are discussed. 1 instruments and reagents UV-1500PC ultraviolet spectrophotometer; electronic balance; sulfuric acid (; anhydrous ethanol; berberine hydrochloride reference substance (China National Institute for the Control of Pharmaceutical and Biological Products); Weikangning tablets (each containing berberine hydrochloride 0.15 g , Sucralfate 0.05 g.2 Method and result 2.1 Selection of determination wavelength Precision weighed 25 mg of berberine hydrochloride reference substance, added an appropriate amount of absolute ethanol, and dissolved by heating on a water bath at 60 Â° C. The solution was filtered to a capacity of 100 mL. In the bottle, add absolute ethanol to the mark and shake well. Precisely measure 2 mL, place it in a 50 mL volumetric flask, dilute to the mark with 0.05 mol/L sulfuric acid solution, shake well. Weigh the amount of sucralfate according to the prescription ratio. The above treatment was carried out by using 0.05 mol/L sulfuric acid solution as a reference solution and scanning between 200-450 nm wavelength range to record the absorption spectrum. The spectrogram showed that berberine hydrochloride had the maximum absorption at 375 nm. The absorption of the aluminum component in the range of 200-450 nm is constant, and the absorption spectra of the two components are correlated at 375 nm. Therefore, the wavelength of 375 nm is selected as the measurement wavelength of berberine hydrochloride. 2.2 Standard curve drawing precision weighing Dry at 60 Â° C to constant The appropriate amount of berberine hydrochloride reference substance, add appropriate amount of absolute ethanol to dissolve in a 60 Â° C water bath, and dilute to 100 mg / L of the solution. Precisely measure the above solution 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0 The mL was placed in a 25 mL volumetric flask, diluted with 0.05 mol/L sulfuric acid solution to the mark, and shaken. The absorbance was measured at a wavelength of 375 nm using a 0.05 mol/L sulfuric acid solution as a reference solution. The measured data was subjected to linear regression. The regression equation is obtained: C=0.0139A+0.0071, r=0.9999. The results show that the concentration of the solution is in the range of 4-28 mg/L, and the absorbance has a good linear relationship with the concentration. 2.3 Stability 0.05 mol/L sulfuric acid The control solution was stored at room temperature for 2, 4, and 6 months, and the absorbance was unchanged. The absorbance was measured immediately after preparation of the test solution, and there was almost no change in the absorbance measured after 24 h of standing. 2.4 Recovery rate Accurately weighed, about 60g of berberine hydrochloride reference substance dried to constant weight at 60 Â°C, and then add appropriate amount of sucralfate corresponding to the prescribed amount, placed in a 100mL volumetric flask, and heated with anhydrous ethanol in a water bath at 60 Â°C. Dilute to the mark with absolute ethanol for about 10 minutes, shake well. 1mL was placed in a 100 mL volumetric flask, diluted to the mark with 0.05 mol/L sulfuric acid solution, and shaken. The absorbance was measured at a wavelength of 375 nm using 0.05 mol/L sulfuric acid solution as a reference solution, and calculated according to the regression equation. The average recovery was 99.55%, and the RSD was 0.36% (n=5). 2.5 Effect of Excipients on Content Determination After the excipient starch and methylol starch in Weikangning tablets were formulated according to the process requirements Weigh the equivalent of 2 tablets of Weikangning tablets in a 250 mL volumetric flask, add an appropriate amount of absolute ethanol, heat in a water bath at 60 Â° C for about 10 minutes to dissolve, add absolute ethanol to the mark, shake well , filtered. Precisely take 5mL of the filtrate and add 0.05mol/L sulfuric acid solution to the 100mL volumetric flask to the mark and shake. The absorbance was measured in the range of 260 to 450 nm, and as a result, there was no absorption and the measurement was not disturbed. 2.6 Determination of sample content In 5 batches of samples, take 20 tablets of Weikangning tablets in each batch, grind finely and mix well. Weigh accurately and weigh an appropriate amount (about 0.15 g of berberine hydrochloride), and dissolve it in a 60 Â° C water bath with an appropriate amount of absolute ethanol. Place the solution in a 100 mL volumetric flask, dilute to the mark with absolute ethanol, and shake well. Accurately pipette 1 mL into a 100 mL volumetric flask, dilute to the mark with 0.05 mol/L sulfuric acid, and shake well. The absorbance was measured at 375 nm with a 0.05 mol/L sulfuric acid solution as a reference solution, and the concentration was calculated according to a regression equation. Key words: UV spectrophotometry; content of berberine hydrochloride in Weikangning tablets; ;</p> </div> </div> <div class="tech-detail-share" >  <div class="bdsharebuttonbox"> <a href="#" class="bds_qzone" data-cmd="qzone" title="Share to QQ Space"></a > <a href="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Weibo"></a> <a href="#" class="bds_weixin" data-cmd= "weixin" title="Share to WeChat"></a> <span>Share to:</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, " bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share": {} }; with (document) 0[(getElementsByTagName($

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Determination of Berberine Hydrochloride in Weikangning Tablets by UV-1500PC Ultraviolet Spectrophotometry - Master's thesis - Dissertation

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